Antioxidant, antimicrobial, and preliminary phytochemical constituents of fermented seeds of <i>Trigonella foenum-graecum</i>

Trigonella foenum-graecum (Fenugreek) is among the earliest plants used as spices and medicine worldwide. This study determined antioxidant activities of fermented seeds T. by methods, ABTS 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonicacid) DPPH (2,2-diphenyl-1-picrylhydrazyl). The phytochemical compounds, pH, in vitro agar well (antimicrobial) assay sample was tested at three concentrations (100%, 50%, 25%) against selected pathogens (Staphylococcus aureus, Salmonella typhi, Escherichia coli) diffusion method. There a pH decline from 5.6 (on day 1) to 3.53 7) finally 3.38 14). property 14th-day revealed free radical scavenging potential correspondence with concentrations. Flavonoids, phenol, phytosterols, terpenoids, alkaloids, tannins were detected samples for 7 14 days, respectively. zones inhibition (in diameter) tune concentrations, this ranged between 6mm – 15mm on 6mm-17mm test pathogens. Hence, validates antimicrobial, activity foenumgraecum supports its use an herbal therapeutic agent diseases associated pathogen.